Method (adapted from APHA Standard Method 3500-Cu C. Bathocuproine Method)Cuprous ion forms a water-soluble orange colored chelate with bathocuproine disulfonate. The absorbance is measured at 484 nm. - 50 mL sample add 1 mL HCl (1:1) + 5 mL hydroxylamine hydrochloride solution + 5 mL sodium citrate + 5 mL disodium bathocuproine solution. Mix well.
- Read absorbance against a blank at 484 nm.
Reagents from Labchem
Reagent | Catalog # | Cost | Shelf life | | Hydroxylamine hydrochloride, 10% | LC155301 | $37.80 for 500 mL | 24 months | | Sodium citrate, 30% | LC236602 | $138.90 for 500 mL | 24 months | | Disodium Bathocuproine, 0.1% | LC136851 | $180.60 for 500 mL | 24 months | | Copper standard, 1 mL = 1 mg Cu | LC134007 | $17.50 for 125 mL | 24 months |
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 Updating... Ċ Joanne Long, Mar 26, 2013, 11:31 AM
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