Copper

Method (adapted from APHA Standard Method 3500-Cu C. Bathocuproine Method)

Cuprous ion forms a water-soluble orange colored chelate with bathocuproine disulfonate. The absorbance is measured at 484 nm.
  1. 50 mL sample add 1 mL HCl (1:1) + 5 mL hydroxylamine hydrochloride solution + 5 mL sodium citrate + 5 mL disodium bathocuproine solution. Mix well.
  2. Read absorbance against a blank at 484 nm.

Reagents from Labchem


 Reagent

 Catalog #

 Cost

 Shelf life

Hydroxylamine hydrochloride, 10% LC155301 $37.80 for 500 mL 24 months
Sodium citrate, 30% LC236602 $138.90 for 500 mL 24 months
Disodium Bathocuproine, 0.1% LC136851 $180.60 for 500 mL 24 months
Copper standard, 1 mL = 1 mg Cu LC134007 $17.50 for 125 mL 24 months

Ċ
Joanne Long,
Mar 26, 2013, 11:31 AM
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