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Hach FerroVer Iron Reagent

A. Reagents and Equipment


Vendor     

 Vendor

 Catalog #

 Price

 Shelf life

FerroVer® Iron Reagent Powder Pillows, 5 mL Hach  92799 $17.59 for 100 tests  
Iron standard, 200 ppm Labchem LC157207 $22.90 for 125 mL  24 months
Distilled water        



B. Iron-Hach Calibration Curve

  • Dilute the 200 ppm iron standard to yield a 10 ppm working stock. 
  • Dilute the working stock as outlined in the Table below:


Tube

Iron Concentration

(ppm)

Volume of 10 ppm working stock (mL)


Volume of distilled water (mL)
1 0.00 0.00 5.0
2 1.25 0.625 4.375
3 2.5 1.25 3.75
4 3.75 1.875 3.125
5 5 2.5 2.50
6 6.25 3.125 1.875
7 7.5 3.75 1.25
8 8.75 4.375 0.625
9 10 5.0 0.0


  • To each 5 mL sample, add the contents of one FerroVer iron reagent powder pillow, shake and let stand. Below is an image of all of the calibration solutions after adding the powder reagent.

  • Transfer approx. 1mL of each calibration solution to a cuvette.
  • In the colorimeter plotting program, select the blue LED and ppm as units. Calibrate the colorimeter with the 0 ppm iron solution and measure absorbance of all of the solutions in duplicate. 

Results

Calibration curve measurements were repeated three times with 3 new working stock dilutions. Data for each of the three separate calibration trials are attached at the bottom of the page (hach_trial1.txt etc). To view this data, download the files and open them in the colorimeter plotting software as described here

An image of the data from one of the calibration curves is also shown below. 





Additional Commercial Test Kits (not currently tested) 


Vendor

Reagent

 Range

Catalog #

Cost

Hach Ferrous Iron Reagent Powder Pillows, pk/100    103769  $24.05 for 100 tests


ċ
hach_trial1.txt
(0k)
Joanne Long,
Apr 23, 2013, 6:59 PM
ċ
hach_trial2.txt
(0k)
Joanne Long,
Apr 23, 2013, 6:59 PM
ċ
hach_trial3.txt
(0k)
Joanne Long,
Apr 23, 2013, 6:59 PM
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