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Hach SulfaVer 4 Sulfate Reagent

A. Reagents and Equipment


Vendor     

 Vendor

 Catalog #

 Price

 Shelf life

SulfaVer® 4 Sulfate Reagent, 5 mL Hach  2106769 $28.95 for 100 tests
Sulfate standard, 1000 ppm Labchem LC255101 $33.20 for 500 mL  24 months
Distilled water        


B. Sulfate-Hach Calibration Curve

  • Dilute the 1000 ppm sulfate standard as outlined in the Table below:


Tube

Sulfate Concentration

(ppm)

Volume of 1000 ppm standard (mL)


Volume of distilled water (mL)
1 0.00 0.00 10.0
2 37.5 0.375 9.625
3 75 0.75 9.25
4 112.5 1.125 8.875
5 150 1.5 8.50
6 187.5 1.875 8.125
7 225 2.25 7.75
8 262.5 2.625 7.375
9 300 3.0 7.0


  • To each 10 mL sample, add the contents of one SulfaVer 4 Sulfate reagent, shake and let stand. Below is an image of all of the calibration solutions after adding the powder reagent.

  • Transfer approx. 1mL of each calibration solution to a cuvette.
  • In the colorimeter plotting program, select the green LED and ppm as units. Calibrate the colorimeter with the 0 ppm sulfate solution and measure absorbance of all of the solutions in duplicate or triplicate. 

Results

Calibration curve measurements were repeated three times. Data for the three separate calibration trials were compiled into one file which is attached at the bottom of the page (sulfate_hach.txt). To view this data, download the file and open in the colorimeter plotting software as described hereThe final plot for all three sets of data is shown below: 


ċ
sulfate_hach.txt
(1k)
Joanne Long,
Jun 12, 2013, 3:17 PM
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